Basic HTML Version
Poster Full Abstracts - Regulation of Gene Expression
Poster board number is above title. The first author is the presenter
347
identified
CG31111
as a potential orthologue of NIAM and designed it
dNIAM
. Both human and fly proteins possess an N-terminal lysine-rich region and C-
terminal phenylalanine/tyrosine-rich (FYRN/FYRC) domains, which are exclusively found in proteins that modify chromatin structure. Knock-down of
dNIAM in the developing eye caused over-proliferation of eye tissue, demonstrating an anti-proliferation function. Analysis of transgenic flies expressing a
dNIAM-GFP showed that the protein localizes to actively transcribed regions of Drosophila polytene chromosomes, including heat shock loci and
developmental puffs. Co-localization studies demonstrated a partial overlap with RNA Polymerase II phosphorylated at serine 5, which occurs at the 5’
region of genes. Collectively, these data demonstrate a conserved anti-proliferation for dNIAM and suggest a role in transcriptional regulation.
808A
Functional characterization of dmyc downstream promoter element in transgenic Drosophila.
Jasmine Kharazmi
1,2
, Cameron Moshfegh
1
. 1) Molec
Biol Lab, Biotech Ctr Zurich, Zurich; 2) University of Zurich-Jrchel, Switzerland.
Myc is a master regulator of growth and proliferation during animal development. Many signals and transcription factors lead to changes in the expression
levels of Drosophila myc, yet no clear model exists to explain the complexity of its regulation at the level of transcription. In this study we used Drosophila
genetic tools to track the dmyc cis-regulatory elements. Bioinformatics analyses identified conserved sequence blocks in the noncoding regions of the dmyc
gene. Investigation of lacZ reporter activity driven by upstream, downstream, and intronic sequences of the dmyc gene in embryonic, larval imaginal discs,
larval brain, and adult ovaries, revealed that it is likely to be transcribed from multiple transcription initiation units including a TATA-less downstream
promoter element in conjunction with an initiator within the intron 2 region (Kharazmi, et.al., Gene Regulation And Systems Biology, 2011, in press). Our
data provide evidence for a modular organization of dmyc regulatory sequences; these modules will most likely be required to generate the tissue-specific
patterns of dmyc transcripts. These data provide a framework for further investigation of the transcriptional regulatory mechanisms of dmyc.