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Poster Full Abstracts - Gametogenesis and Organogenesis
Poster board number is above title. The first author is the presenter
288
spermatogenesis to those in
CG4701
and
nmd
mutants using green fluorescent protein-tagged versions of the proteins. Curiously, we found that mutant flies
with the
β-tubulin-GFP
transgene had a partially rescued phenotype, consistent with the suspected functional connection between Nmd and CG4701 and
microtubules. We are using chemical fluorescent tubulin stains as an alternative for studying true microtubule structure in the mutants. In addition, we are
using RNAi to ask whether gene knockdowns phenocopy
CG4701
and
nmd
mutant alleles. Preliminary data show no effect on spermatogenesis, so we are
examining whether our chosen GAL4 driver (expressed very early in spermatogenesis) in fact drives efficient gene knockdown.